Internships proposal
The UTCBS accompanies and supervises interns all year long
In 2022, we supervised students in BTS in 1st and 2nd year for professional internships, students in 3rd year for observation internship.
Various study topics were covered :
BTS 2 topic: Insertion of the GFP* fluorescent protein coding sequence, upstream of the B-actin* gene, using CRISPR/CAS9* technology, on cultured mouse skin cells. The objective was to show that we were able to use this recent technology (awarded a Nobel Prize in Chemistry in 2020) on a constitutive gene* and on cells that are easy to transfect*.
Common topic for BTS 1: Insertion of the sequence coding for the fluorescent protein GFP, upstream of the B-actin gene, using CRISPR/CAS9 technology, on mouse macrophages* in culture. The objective was to apply to macrophages what had been successfully achieved on skin cells, knowing that macrophages are more difficult to transfect.
3rd grade subject : Identification of a plasmid, byThe objective was to show the different types of plasmids that can be used to identify a plasmid, by bacterial transformation, cell transfection and “genetic fingerprinting”. The objective was to show him different techniques and analysis tools of the laboratory in the playful form of a “police investigation”.
From January 2023, two BTS 2 are working on a common subject: Genetic and protein analysis of macrophages (modified by CRISPR/CAS9 technology) to identify a clone producing the cytokine GFP-TNF*.
February-March 2023: Accompaniment of a BTS 2 whose subject is: Study of the production of the TNF cytokine by macrophages (wild or modified by CRISPR/CAS9 technology) after induction by natural or artificial activating molecules (CRISPR/dCas9 plasmids activating TNF).
Also to read
For the 2nd year running, UTCBS is hosting a delegation of South African researchers as part of a bilateral PHC
For the 2nd year running, UTCBS is hosting a delegation of South African researchers as part of a bilateral PHC
CNRS honors Néovacs and UTCBS, united for a therapeutic RNA vaccine against asthma
Suresnes, August 29, 2024 - 8am CET - Neovacs (Euronext Growth Paris : ALNEV), a preclinical-stage biopharmaceutical company developing novel therapies for inflammatory and autoimmune diseases, is honored to be listed among the latest CNRS publications following the...
Publication in Advanced Science of Preclinical tests obtained with a pFAR plasmid
Gene Electrotransfer via Conductivity-Clamped Electric Field Focusing Pivots Sensori-Motor DNA Therapeutics: “A Spoonful of Sugar Helps the Medicine Go Down” https://doi.org/10.1002/advs.202401392
Khair Alareth, Caroline Roques and Nasir Arafath with Nathalie Mignet to present UTCBS’s work at the Controlled release society international congress in Bologna.
UTCBS presents its work at the international congress of the "controlled release society" in Bologna. The UTCBS team has shown the interest of nanoparticles both for the imaging of ROS in vivo and the promotion of antioxydant therapeutic activity. Caroline Roques...