Genetic immunization

The aim of this project is to design mRNA vectorization strategies for vaccine applications against infectious diseases. The antigen against which the immune response is sought is encoded in the in vitro transcribed mRNA molecule (IVT-mRNA).

 

Schematic representation of a synthetic mRNA encoding a multi-epitope antigen

 

Vectorization strategies are required to give mRNA access to the cellular machinery, enabling in situ production of the antigen and the onset of the immune response against it. Unlike plasmid DNA, mRNA does not need to be transported to the nucleus, as it is translated in the cytoplasm. We intend to formulate mRNA-IVT molecules in our self-assembling nanoparticle vectors and administer them via different routes to assess the humoral and cellular immune response induced by expression of the mRNA-IVT-encoded antigen. Two vaccine applications are envisaged: (i) Immunization against Chagas disease in collaboration with Dr Alonso-Padillala and Pr Gascón, University of Barcelona, (ii) Immunization against gestational malaria in collaboration with Dr Ndam, MERIT laboratory “Mère et enfant avec infections tropicales”, IRD University of Paris.